L2 454 alignments

Track ID=L2.ws220.sam_sorted [Download entire dataset as BAM or BigWig]

3RACE polyA-capture N2 L2 454 alignments (Piano project, Kim subgroup)

Details

3UTRs were isolated from L2 larva of C. elegans N2 worms using a poly-A capturing technique. RNAs were sequenced on the Roche 454 platform. This submission reflects the alignments of the deconvoluted, L2 stage-specific reads to WS190 genome using BLAT.

General Description

The 3 untranslated region (3UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3RACE cloning-sequencing stragety to identify the 3UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.

Protocols

  1. Data Analysis: BLAT alignment
  1. External Links: GSM446653

Related modENCODE submissions:


Release Date: 2009-11-06