daf-9 Dauer 454 alignments

Track ID=L33daf09.ws220.sam_sorted [Download entire dataset as BAM or BigWig]

3'RACE polyA-capture daf-9 dauer 454 alignments (Piano project, Kim subgroup)

Details

3'UTRs were isolated from dauer larva of C. elegans daf-9 worms using a poly-A capturing technique. RNAs were sequenced on the Roche 454 platform. This submission reflects the alignments of the deconvoluted, dauer stage-specific reads to WS190 genome using BLAT.

Series Description

The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.

Protocols

  1. Data Analysis: BLAT alignment
  1. External Links: GSM446660

Related modENCODE submissions:


Release Date: 2009-11-06