Centromere Specificaton ChIP-chip arraysCenromere Specificaton and Kinetochore Function (Lieb project, Desai subgroup)General DescriptionSynchronized C. elegans early embryos from strain N2 were treated with the cross-linking reagent formaldehyde. Sonicated chromatin was prepared and immunoprecipitated with an affinity-purified polyclonal antibody that recognizes the centromere-binding protein HCP-3 (CENP-A). After whole genome amplification, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down in the ChIP. Normalized log2(ratios) are shown in this track.Protocols
Experimental Reagents
Sample Details
Release Date: 2009-10-15 |