Histone Modifications (H3K79) ChIP-chip arrays
Lieb AB3594_H3K79ME2346021_N2_MXEMB (Lieb project, Lieb subgroup)Synchronized C. elegans mixed embryos from strain N2 were treated with the cross-linking reagent formaldehyde. Sonicated chromatin was prepared and immunoprecipitated with an affinity-purified polyclonal antibody that recognizes histone H3 dimethylated on lysine 79. After whole genome amplification, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down in the ChIP. Normalized log2(ratios) are shown in this track.
General DescriptionThe focus of our analysis will be elements that specify nucleosome
positioning and occupancy, control domains of gene expression, induce
repression of the X chromosome, guide mitotic segregation and genome
duplication, govern homolog pairing and recombination during meiosis,
and organize chromosome positioning within the nucleus. Our 126
strategically selected targets include key histone
modifications and histone variants.
We will integrate information generated with existing knowledge on the
biology of the targets and perform ChIP-chip analysis on mutant and RNAi
extracts lacking selected target proteins.