Mononucleosomes

citation =

Mononucleosomes_JK1107_AD ( Lieb project, Segal subgroup)

Germlineless young adults from JK1107 strain were treated with the cross-linking agent formaldehyde and chromatin were digested with micrococcal nuclease. After purification, mononucleosome size-selected DNA fragments were subjected to deep sequencing using paired end reads on an Illumina GA-2 platform. This signal intensity track shows the sequence tag density aligned across the genome for one biological replicate.

Protocols

  1. Growth and isolation: Worm germlineless adult soma growth and harvest:JL:5
  2. Sample preparation: Solexa Library Preparation:JL:SE1
  3. Data Analysis: Nucleosome sequencing analysis:JL:1, Nucleosome sequencing normalization:JL:1
  4. Other Protocols: Worm adult nucleosome preparation:JL:SE1
  1. Growth Conditions: 20

Sample Details

  1. Animals/Lines: Germlineless young adult, JK1107, seq-mononucleosome_JK1107_adult, seq-mononucleosome_JK1107_adult
  2. External Links: GSE20136


Release Date: 2010-02-15