early embryo 50-120 (initial reads)

C. elegans N2 RNA-Seq Early Embryo (50 hours post L1, embryo +120 minutes) sequences and alignments

General Description

Paired-end RNA-seq was performed on Early Embryo samples isolated from gravid adults 50 hours post L1 and incubated an additional 120 minutes. Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.

Protocols

  1. Growth and isolation: Worm_growth, Staging_and_isolation, RNA_isolation
  2. Sample preparation: PE_Sequencing
  3. Other Protocols: Alignment


Release Date: 2011-08-24