EFL-1 Combined (GFP ChIP)

Identification of Transcription Factor EFL-1::GFP Binding Regions in L1 (Snyder project, Snyder subgroup)

Details

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General Description

We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.

Protocols

  1. Growth and isolation: Plated Worm Growth and Harvest
  2. Sample preparation: ChIP, Illumina Deep Sequencing
  3. Data Analysis: Illumina Data Merging, Skip Illumina Data Merging
  4. Other Protocols: Illumina Data Analysis, Peak Calling, Illumina Data Analysis, ChIP-seq replicate verification

Experimental Reagents

    Antibodies:

Sample Details

    Animals/Lines:


Release Date: 2013-05-06 Submission 3211