ALR-1 Combined (GFP ChIP)
Identification of Transcription Factor ALR-1::GFP Binding Regions in L2 (Snyder project, Snyder subgroup)
General DescriptionSynchronized L2 larvae from C. elegans strain OP200 (a transgenic strain engineered to express a gene fusion between alr-1 and GFP) were treated with the cross-linking reagent formaldehyde. After lysis and sonication, the chromatin was immunoprecipitated with an affinity-purified antibody that recognizes GFP. The bound DNA was purified and sequenced in an Illumina GA-2. A sample of the input DNA was sequenced in parallel. The ChIP-seq data generated by this experiment was analyzed using the PeakSeq peak-calling algorithm to predict protein binding sites in the C. elegans genome.
Release Date: 2009-11-26 Track 3156