PES-1 Combined (GFP ChIP)

Identification of Transcription Factor PES-1::GFP Binding Regions in L4 (Snyder project,Snyder subgroup)

General Description

We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is tagged with the same GFP fusion protein, permitting validation of the gene's correct spatio-temporal expression pattern in transgenic animals. Chromatin immunoprecipitation on each strain is peformed using an anti-GFP antibody, and any bound DNA is deep-sequenced using Solexa GA2 technology.

Protocols

1. Growth and isolation: Worm Growth and Harvest
2. Sample preparation: Illumina Deep Sequencing, ChIP
3. Data Analysis: Illumina Data Merging, Skip Illumina Data Merging, Illumina Data Analysis, Peak Calling

Experimental Reagents

Growth Conditions:
1. Antibodies: Anti-eGFP

Sample Details

1. Animals/Lines: L4

Release Date: 2009-11-28