Adult spe-9 Day 8

spe-9 adult hermaphrodite 8 days post-L4 small ncRNA RNA-Seq (Waterston project, Slack subgroup)

Details

small RNAs (less than 200nt) were isolated from stress tested Day8 spe-9 23dC animals, and sequenced using Illumina technology. Sequences were aligned to the WS190 genome using SOAP, converted to SAM, then lifted to WS220. Tracks show alignments/read density.
Total read count: 10263861
Mapped read count: 14194641
Alignment rate: 138%

General Description

We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.

Protocols

  1. Growth and isolation: worm growth, worm staging and isolation, small RNA isolation
  2. Sample preparation: cDNA library prep and Illumina sequencing
  3. Other Protocols: SOAP alignment
  1. External Links: GSM463027, GSM463030, SRR032281, SRR032284


Release Date: 2012-05-16