him-8

Track ID=miRNA_spermatids.ws220.sam.gz_sorted [Download entire dataset as BAM or BigWig]

miRNA isolation and 454 sequencing from him-8 male spermatids (Piano project, Kim subgroup)

Detail

small RNAs (<200 bp) were isolated from him-8 spermatids and sequenced on the Roche 454 platform, and aligned to the WS190 genome using MegaBlast.

Series Description

The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.

Protocols

  1. Growth and isolation: Worm growth, Spermatid extraction from whole males, Total RNA extraction from worm tissues, CDNA library preparation for 18-32 nt small RNA, small RNA library prep for 454 sequencing
  2. Sample preparation: 454 deep sequencing
  3. Data Analysis: megaBLAST alignment
  1. Growth Conditions: 25

Sample Details

  1. Animals/Lines: Adult male 20dC 70hr post-L1, spermatids, CB1489

Release Date: 2010-02-12