him-8

Track ID=miRNA_spermatids_solexa.ws220.sam.gz_sorted [Download entire dataset as BAM or BigWig]

miRNA isolation and Illumina sequencing from him-8 male spermatids (Piano project, Kim subgroup)

Detail

small RNAs (<200 bp) were isolated from spermatids from him-8 male worms and sequenced on the Illumina platform, and aligned to the WS190 genome using MegaBlast.

Series Description

The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.

Protocols

1. Growth and isolation: Worm growth, Spermatid extraction from whole males, Total RNA extraction from worm tissues, CDNA library preparation for 18-32 nt small RNA, small RNA library prep for Illumina sequencing
2. Sample preparation: Illumina deep sequencing
3. Data Analysis: megaBLAST alignment

1. Growth Conditions: 25 degrees

Sample Details

1. Animals/Lines: Adult male 20dC 70hr post-L1, spermatids, CB1489

Release Date: 2010-02-14