N2 whole worms

Track ID=miRNA_young_adult.ws220.sam.gz_sorted [Download entire dataset as BAM or BigWig]

miRNA isolation and 454 sequencing from N2 whole worms (Piano project, Kim subgroup)

Detail

small RNAs (<200 bp) were isolated from N2 whole worms and sequenced on the Roche 454 platform, and aligned to the WS190 genome using MegaBlast.

Series Description

The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.

Protocols

  1. Growth and isolation: Worm growth, Total RNA extraction from whole worms, CDNA library preparation for 18-32 nt small RNA, small RNA library prep for 454 sequencing
  2. Sample preparation: 454 deep sequencing
  3. Data Analysis: megaBLAST alignment
  1. Growth Conditions: 25 degrees celsius

Sample Details

  1. Animals/Lines: young Adult 25dC, N2

Release Date: 2010-02-12