Utrome minipool 2

Encyclopedia of C. elegans 3' UTRs and their regulatory elements - 454 sequencing of RACE clones (Piano project, Piano subgroup)

General Description

The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.

Protocols

  1. Growth and isolation: Worm growth, Total RNA extraction from whole worms, Minipool library preparation
  2. Sample preparation: 454 deep sequencing
  3. Other Protocols: LASTZ alignment of minipools


Release Date: 2011-01-24