L4 rRNA depleted array capture

RNA-Seq C. elegans N2 L4 rRNA depleted array captured library sequences and alignments

Details

These array capture samples are from C. elegans N2 L4 animals and were treated with a rRNA depletion kit to improve the sample quality.

General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.

Protocols

  1. Growth and isolation: RNA_isolation, Staging_and_isolation, Worm_growth
  2. Sample preparation: PE_Sequencing
  3. Other Protocols: ribozero, Normalization, Alignment
  1. External Links: SRR136598


Release Date: 2012-01-25 Submission 4173