dauer daf-2 combined replicates

C. elegans daf-2(e1370) Dauer combined replicates sequences and alignments

General Description

RNA-seq data for C. elegans daf-2 dauers was generated across a series of replicated experiments. All the replicates were combined and merged to create a single C. elegans daf-2 dauer dataset of coverage and alignments. Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages, tissues and conditions of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts. We then provide lists of evidence of level of expression per transcript and catalogs of confirmed splice junctions, trans-spliced leader sequences, start sites, end sites, and poly-adenylation tracts for each stage/tissue/condition.

Protocols

  1. Growth and isolation: Worm_growth, Staging_and_isolation, RNA_isolation
  2. Sample preparation: PE_Sequencing
  3. Other Protocols: Alignment, Merge, Normalization
  1. External Links: SRR027187, and 191 more.


Release Date: 2012-02-08