PA14 12-hr Treatment 1 day post-L4 sncRNA

PA14 12-hr treatment 1 day post-L4 small ncRNA RNA-Seq (Waterston project, Slack subgroup)

Details

small RNAs (less than 200nt) were isolated from stress tested stress PA14 animals, and sequenced using Illumina technology. Sequences were aligned to the WS190 genome using SOAP, converted to SAM, then lifted to WS220. Tracks show alignments/read density.
Total read count: 17744396
Mapped read count: 5569562
Alignment rate: 31%

General Description

We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression under various stress conditions in C. elegans. RNAs were prepared C. elegans hermaphrodites, under starvation, bacterial exposure, and heat shock stresses, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa.

Protocols

  1. Growth and isolation: worm growth, worm staging and isolation, small RNA isolation
  2. Sample preparation: cDNA library prep and Illumina sequencing
  3. Other Protocols: PA14 exposure, SOAP alignment
  1. External Links: GSM632212, SRR077256


Release Date: 2012-05-18