DauerDAF2

Celegans Dev Timecourse Expression DauerDAF2 RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages and strains of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.

Protocols

  1. Growth and isolation: Library preparation for Illumina Sequencing, Staging_and_isolation, RNA_isolation, polyA Selection, Worm_growth
  2. Sample preparation: Illumina paired-end sequencing on HiSeq2000, cDNA amplification with random hexamers for sequencing, Illumina single-end sequencing on GAIIx
  3. Other Protocols: Normalization, Alignment
  1. External Links: SRR027187


Release Date: 2013-11-05 Submission 6534