Celegans Dev Timecourse Expression DauerEntryDAF2 RNA-seq sequences and alignment (Waterston project, Waterston subgroup)


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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages and strains of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.


  1. Growth and isolation: Library preparation for Illumina Sequencing, Staging_and_isolation, RNA_isolation, polyA Selection, Worm_growth
  2. Sample preparation: cDNA amplification with random hexamers for sequencing, Illumina paired-end sequencing on HiSeq2000, Illumina single-end sequencing on GAIIx, Illumina single-end sequencing on HiSeq2000
  3. Other Protocols: Normalization, Alignment
  1. External Links: SRR027189

Release Date: 2013-11-05 Submission 6535