N2_4cell_EE_DSN-Negative-Positive

Celegans Dev Timecourse Expression N2_4cell_EE_DSN-Negative-Positive RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages and strains of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.

Protocols

  1. Growth and isolation: Staging_and_isolation, RNA library preparation of hand-picked embryos, RNA_isolation, Worm_growth
  2. Sample preparation: cDNA amplification with random hexamers for sequencing, Illumina single-end sequencing on GAIIx
  3. Other Protocols: Normalization, Alignment, rRNA removal with DSN treatment
  1. External Links: SRR316755, SRR316756, SRR316754


Release Date: 2013-11-06 Submission 6548