N2_4cell_EE_RZ-56

Celegans Dev Timecourse Expression N2_4cell_EE_RZ-56 RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

Place a submission-specific statement in here.

General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages and strains of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.

Protocols

  1. Growth and isolation: Staging_and_isolation, RNA library preparation of hand-picked embryos, RNA_isolation, Worm_growth
  2. Sample preparation: Illumina paired-end sequencing on HiSeq2000, cDNA amplification with random hexamers for sequencing
  3. Other Protocols: rRNA removal with RiboZero, Normalization, Alignment
  1. External Links: SRR493100, SRR493099


Release Date: 2013-11-06 Submission 6549