N2_EE_DSN-51

Celegans Dev Timecourse Expression N2_EE_DSN-51 RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages and strains of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.

Protocols

  1. Growth and isolation: Worm_growth, Staging_and_isolation, RNA_isolation, Library preparation for Illumina Sequencing
  2. Sample preparation: cDNA amplification with random hexamers for sequencing, Illumina paired-end sequencing on HiSeq2000
  3. Other Protocols: rRNA removal with DSN treatment, Alignment, Normalization
  1. External Links: SRR493105, SRR554453


Release Date: 2013-11-06