N2_LE-2cap6

Celegans Dev Timecourse Expression N2_LE-2cap6 RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages and strains of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.

Protocols

  1. Growth and isolation: Worm_growth, Staging_and_isolation, RNA_isolation, Array Capture, Library preparation for Illumina Sequencing
  2. Sample preparation: cDNA amplification with random hexamers for sequencing, Illumina single-end sequencing on GAIIx
  3. Other Protocols: Alignment, Normalization
  1. External Links: SRR136599


Release Date: 2013-11-06