N2-EEcap5

Celegans Dev Timecourse Expression N2-EEcap5 RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from various stages and strains of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.

Protocols

1. Growth and isolation: Library preparation for Illumina Sequencing, Array Capture, Staging_and_isolation, RNA_isolation, Worm_growth
2. Sample preparation: cDNA amplification with random hexamers for sequencing, Illumina single-end sequencing on GAIIx
3. Other Protocols: Normalization, Alignment

1. External Links: SRR089795, SRR089796

Release Date: 2013-11-06 Submission 6559