early embryo 50-600

Celegans Emb Timecourse Expression N2_EE_50-600 RNA-seq sequences and alignment (Waterston project, Waterston subgroup)

Details

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General Description

Using massively parallel sequencing by synthesis methods, we are surveying transcripts from 30 minute intervals of embryonic development of the nematode C. elegans. We use novel statistical approaches to evaluate coverage of annotated features of the genome and of candidate processed transcripts.

Protocols

1. Growth and isolation: Worm_growth, Staging_and_isolation, RNA_isolation, polyA Selection, Library preparation for Illumina Sequencing
2. Sample preparation: cDNA amplification with random hexamers for sequencing, Illumina paired-end sequencing on HiSeq2000, Illumina single-end sequencing on HiSeq2000
3. Other Protocols: Alignment, Normalization

1. External Links: SRR350990, SRR350991, SRR350992, SRR350993, SRR359088 , and 5 more.

Release Date: 2012-10-19